My recent research

Updated at Mar. 18, 2001

I am an Assistant Professor in Department of Pharmacology, School of Medcine, Kumamoto University, JAPAN. My research is that the role of intracellular ion activities in pH regulation of cardiac muscle in physiological or ischemic condition with electrophysiological methods including ionic selective microelectrode and patch clamp recording. Recently, I take my interest in research for intracellular anion and proton activities during ischemic and reperfusion injury and effects of stilbene derivatives, SITS and DIDS on development of intracellular acidosis during ischemia in isolated guinea pig ventricular papillary muscle in vitro were examined. This work is supported by Grant-in-Aid from the Japanese Ministry of Education, Science and Culture and won "The JJP Prize 1997 Awards" .

DO YOU KNOW the role of Na + in T cell activation?

A putative model for the role of the amiloride-sensitive and TTX-resistant Na+ channel in the T cell activation. Our results suggest that engagement of TCR with the MHC-peptide complex would directly activate or remove the "shutter" of a voltage-dependent, amiloride-blockable, but TTX-resistant, Na+ channel presented in resting T cells. Activation of this Na+ channel then induces influxes of Na+. The increased [Na+ ]i may enhance the Na+ -Ca2+ exchange in plasma membrane with a reverse model or stimulate the Na+ -Ca2+ exchange in mitochondrial membrane to result in a sustained increase in [Ca2+ ]i. Therefore, the TCR stimulation-induced Ca2+ response may, at least, involve two important pathways: 1) TCR stimulation induces an increase in inositol trisphosphate and Ca2+ release from Ca2+ stores, then triggers activation of calcium release-activated calcium channels (ICRAC; see text); and 2) TCR stimulation removes the shutter of Na+ channels and induces an increase in [Na+ ]i. The elevated [Na+ ]i may enhance the operation of the reverse Na+-Ca2+ exchange in plasma membrane to increase Ca2+ influx or trigger the Na+ -Ca2+ exchange in mitochondrial membrane to promote Ca2+ release from mitochondrial stores. (See The Jounal of Immunology. 2000; 165:83-90)

My recent publications:

1. Lai ZF, and Nishi K.Enhancement of intracellular Cl- concentrations induced by extracellular ATP in guinea pig ventricular muscle. Jpan J Pharmacol. 84(4):438-448. 2000.[The PDF file type of this paper]
2. Lai Z.-F., Chen Y.-Z., Nishimura Y. and Nishi K.: An amiloride sensitive and voltage-dependent Na+-channel in an HLA-DR-restricted human T cell clone. The Jounal of Immunology. 2000; 165:83-90.[The PDF file type of this paper]
3. Chen YZ, Lai ZF, Nishi K and Nishimura Y. Modulation of calcium response patterns by altered peptide ligands in a human T cell clone. Eur. J. Immunol. 28(12):3929-3939;1998. [See here.][ See related articles] and[The PDF file type of this paper]
4. Lai ZF, and Nishi K. Intracellular chloride activity increases in guinea pig ventricular muscle during simulated ischemia. Am. J. Physiol.275 (Heart Circ. Physiol. 44): H1613-H1619, 1998; [See here][See related articles][The PDF file type of this paper]
5. Liu J, Lai ZF, Wang XD, Tokutomi N and Nishi K. Inhibition of sodium current by chloride channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in guinea pig cardiac ventricular cells. J Cardiovascu Pharmacol . 31(4):558-567;1998.[See related articles],[The PDF file type of this paper]
6. Sato D, Lai ZF,Tokutomi N, Tokutomi Y, Maeda H, Nishikawa S, Nishikawa SI*, Ogawa M and Nishi K. Impairment of Kit-dependent development of interstitial cells alters contractile responses of the murine intestinal tract Am J Physiol 1996: 271(Gastrointest. Liver Physiol. 34)G761-G771. also you can directly see it by pushing your mouse in here, or , here; and [See related articles],[The PDF file type of this paper]
7. Lai ZF, Jie Liu and Katsuhide Nishi .Effects of stilbene derivatives SITS and DIDS on development of intracellular acidosis during ischemia in isolated guinea pig ventricular papillary muscle in vitro Jpan J Pharmacl 72(2):161-174,1996 Oct.or see: here; and full text in: here; and [See related articles][The PDF file type of this paper]
8. Nishi K, Tokutomi N, Sato D, Tokutomi Y, Sugita M, Lai ZF, Torihashi S. Identification of pacemaker cells of the Gastrointestinal tract and their functional significanc. Gastrointestinal Function. Regulation and disturbances. Excerpta Medica Ltd. Tokyo, 14:27-43;1996.
9. Lai ZF. Hotokebuchi N. Cragoe EJ Jr. Nishi K. Effects of 5-(N,N-hexamethylene)amiloride on action potentials, intracellular Na, and pH of guinea pig ventricular muscle in vitro. Journal of Cardiovascular Pharmacology. 23(2):259-67, 1994; and [See related articles], [The PDF file type of this paper]
10. Lai ZF. Cao Q. Chen S. Han Z. Role of amygdaloid nucleus in the correlation between the heart and the acupoint neiguan in rabbits. Journal of Traditional Chinese Medicine. 11(2):128-38, 1991. [See related articles][The PDF file type of this paper]
11. Chen S. Zhu Q. Ju H. Hao J. Lai ZF. Zou C. Zhang W. Zhao S. Chen X. Zhang H. et al. The role of oxygen free radicals in myocardial ischemia/reperfusion injury. [Journal Article] Chinese Medical Sciences Journal. 6(3):127-31, 1991. [See related articles][The PDF file type of this paper]
12. Lar ZF, Cao QS. Chen SP. Han ZJ. Effect of electro-acupuncture of "Neiguan" on spontaneous discharges of single unit in amygdaloid nucleus in rabbits. Journal of Traditional Chinese Medicine. 9(2):144-50, 1989. [ See related articles][The PDF file type of this paper]
13. Lai ZF,Chen SG and Zhang H: Effect of active oxygen on contraction in papillary muscle of guinea pig heart. J Chin Circulation 7(1):58-60. 1992.
14. Lai ZF, Chen SG, Zhao SH: Effect of extrnal free radicals generating system on electrophysiologic characteristics of rat's papillary muscle in ventricle. Chin J Pathophysiol. 7(1):58-62;1991.[The PDF file type of this paper]
15. Lai ZF, Ca0 QS, Chen SP and Han ZJ: Effect of acute myocardial ischemia on the discharges of single units in amygdaloid complex in rabbits. J Chin Circulation 4(2); 166-167; 1989
16. Review: Nishi K. and Lai Z.F. (in Japanese): Changes in intracellular cation concentrations in cardiac muscle and its physiological significance. Jpn J Cardiac Pacing Electrophysiol 9(2):102-110;1993.
17. Lai ZF. Relationship between changes in intracellular chloride ions and arrhythmias induced by ischemia and reperfusion in guinea pig ventricular papillary muscle. J Kumamoto Medical Association. 70(2-3):222-223; 1996.
18. Lai ZF and Nishi K. Change of slow waves induced by anti-kit monoclonal antibody in smooth muscle cells of neonatal mouse. J Smooth Muscle Res 31(5):305-306; 1995.
19. Lai ZF, Cao QS, Chen SP and Han ZJ: Effect of acute myocardial ischemia on the discharges of single units in amygdaloid complex in rabbits. J. Chin. Circulation 4(2); 166-167; 1989

20. Lai ZF , Cao QS, Chen SP and Han ZJ. Electroacupuncture at "Neiguan" for the experimental myocardiac ischemia in rabbits: Role of amygdaloid complex. Article on acupuncture and moxibustion. Dedicated to the Confederation of World Foundation of Acunpuncture and Moxibustion (WFAS) and first convention of WFAS . Ed. by CAAM, Beijing, 1987:437-438.

21. Lai ZF, Wang X and Nishi K. Enhancement of spontaneous electrical activities and intracellular chloride concentrations induced by extracellular ATP in isolated guinea pig ventricular muscle. J Mol Cell Cardiol. Submitted
22. Review: Lai ZF. Current perspective: TENS treatment of angina pectorisi. Abroad Medicine. 44-46, 1986.

23. Lai ZF, Cao QS, Chen SP and Han ZJ. Electroacupuncture at "Neiguan" for the experimental myocardiac ischemia in rabbits: Role of amygdaloid complex. Article on acupuncture and moxibustion. Dedicated to the Confederation of World Foundation of Acunpuncture and Moxibustion (WFAS) and first convention of WFAS . Ed. by CAAM, Beijing, 1987:437-438.

24. Cao QS, Liu JL and Lai ZF,: An approach to the theory of relationship between acupoints and viscera. II . Role of central nervous system between "Neiguan" and heart. Article on acupuncture and moxibustion. Dedicated to the Confederation of World Foundation of Acupuncture and Moxibustion (WFAS) and first convention of WFAS . Ed. by CAAM, Beijing. 1987; 425-426.

25. Lai Z.F. (Review) Homeostasis of intracellular chloride concentrations during cardiac ischemia and its physiological significance. Acta Academiae Medicinae Jiangxi. 41(2):7-15; 2001.

Summary of the JJP Prize Award paper in Chinese (in GB) or ( The pdf file of my research in Japanese).



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